Cell line/type | At-MSCs (adipose tissue derived mesenchymal stem cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | Mesencult-XF Al-Saqi et al. 2014 |
Al-Saqi, S. H., Saliem, M., Asikainen, S., Quezada, H. C., Ekblad, Å., Hovatta, O., ... & Götherström, C. (2014). Defined serum-free media for in vitro expansion of adipose-derived mesenchymal stem cells. Cytotherapy, 16(7), 915-926. The aim of this study was to evaluate a defined serum-free medium for in vitro expansion of MSCs as a prerequisite for their clinical use. Adipose tissue was isolated from healthy donors. Cells were isolated and expanded for five passages in serum-free medium (Mesencult-XF) and Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (DMEM-FBS). MSC morphology, marker expression, viability, population doubling time and differentiation potential toward osteogenic and adipogenic lineages were evaluated. Bone marrow MSCs were included as controls. Ad-MSCs cultured in Mesencult-XF had shorter population doubling time (33.3 +/- 13.7 h) compared with those cultured in DMEM-FBS (54.3 +/- 41.0 h, P < 0.05). Ad-MSCs cultured in Mesencult-XF displayed a stable morphology and surface marker expression and a higher differentiation potential in comparison to Ad-MSCs cultured in DMEM-FBS. Mesencult-XF medium is a commecial product. The precise formulation is proprietary. https://www.sciencedirect.com/science/article/pii/S1465324914004526 |
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Source | Literature - commercial |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |
Antibiotics free > Yes | Yes |