Cell line/type | CD34+ |
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Species | Human |
Animal free | Unspecified |
Product | StemBioA medium Cheung et al. 2007 |
Cheung, J. O., Casals-Pascual, C., Roberts, D. J., & Watt, S. M. (2007). A small-scale serum-free liquid cell culture model of erythropoiesis to assess the effects of exogenous factors. Journal of immunological methods, 319(1-2), 104-117. DOI: 10.1016/j.jim.2006.11.002 In this study, the authors have developed a serum-free liquid culture model of erythropoiesis using human umbilical cord blood CD34(+) cells cultured in the cytokine combination, interleukin-3 (IL-3), IL-6, stem cell factor (SCF) and erythropoietin (Epo), over 14 days. The results demonstrate a small-scale serum-free liquid culture model of erythropoiesis that is comparable with and complements our well-defined large-scale model. This system would prove useful for screening the effects of exogenous factors on erythropoiesis in vitro. StemBioA was supplemented with different optimized cytokine cocktails, as specified in the Materials and Methods, under the heading; 2.2. Large-scale liquid cell cultures. StemBioA is a commercial product. The formulation is proprietary. https://www.sciencedirect.com/science/article/pii/S0022175906003371 |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |