Cell line/type | BMSCs (bone marrow derived stem cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | Mesencult XF Medium/stemgro hMSC medium |
Hervy, M., Weber, J. L., Pecheul, M., Dolley-Sonneville, P., Henry, D., Zhou, Y., & Melkoumian, Z. (2014). Long term expansion of bone marrow-derived hMSCs on novel synthetic microcarriers in xeno-free, defined conditions. PLoS One, 9(3), e92120. In this study, the long term culture of hMSCs on novel synthetic Synthemax II microcarriers in two different xeno-free media is reported. Cells were maintained over 40 days on sterile, ready-to-use microcarriers in spinner flasks with programmed agitation. hMSC expansion was obtained by addition of fresh beads without the need for enzymatic dissociation. A cumulative cell expansion of .10,000 fold, and cells retained normal hMSC phenotype, karyotype, and tri-lineage differentiation potential was achieved. Mesencult XF Medium and stemgro hMSC medium are both commercial media. The precise formulation is proprietary. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3956887/pdf/pone.0092120.pdf |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Contains phenol red > No | No |