Cell line/type | ASCs (adipose tissue derived stem cells) |
---|---|
Species | Human |
Animal free | Yes |
Product | AR8 Sefcik et al. 2008 |
Sefcik, L. S., Neal, R. A., Kaszuba, S. N., Parker, A. M., Katz, A. J., Ogle, R. C., & Botchwey, E. A. (2008). Collagen nanofibres are a biomimetic substrate for the serumāfree osteogenic differentiation of human adipose stem cells. Journal of tissue engineering and regenerative medicine, 2(4), 210-220. In this study, the authors compared the osteogenic differentiation behavior of human adipose stem cells (ASCs) on a 3D nanofibre matrix of type I rat tail collagen (RTC) and a 2D RTC collagen-coated substrate, using a novel serum-free osteogenic medium. The serum-free medium significantly enhanced the numbers of proliferating cells in culture, compared to ASCs in traditional basal medium containing 10% animal serum, highlighting a potential clinical role for in vitro stem cell expansion. These studies demonstrate that 3D nanoscale morphology plays a critical role in regulating cell fate processes and in vitro osteogenic differentiation of ASCs under serum-free conditions. The ASC serum-free medium formulation (AR8) was developed in the laboratory of Dr. Adam J. Katz. The composition of the media (without concentrations) is listed in the Materials and Methods, under the heading Cell isolation and culture. The formulation of AE8 medium is listed in Table 2. of Parker et al. 2007. |
|
Source | Literature - own formulation |
Chemically defined > Unspecified | Unspecified |
Contains phenol red > Yes | Yes |
Antibiotics free > No | No |