Cell line/type | Cardiac myocytes |
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Species | Rat |
Animal free | Unspecified |
Product | Dhir et al. 2009 |
Dhir, V., Natarajan, A., Stancescu, M., Chunder, A., Bhargava, N., Das, M., ... & Molnar, P. (2009). Patterning of diverse mammalian cell types in serum free medium with photoablation. Biotechnology progress, 25(2), 594-603. In this study, direct photolithographic patterning of two types of multilayers, PAA (poly acrylic acid)/PAAm (poly acryl amide) and PAA/PAH (poly allyl amine hydrochloride), were developed to pattern mammalian neuronal, skeletal, and cardiac muscle cells. Photolithographic patterning of polyelectrolyte multilayers is a simple, versatile, and robust method to pattern cells with exceptional long-term stability and protein adsorption resistance. . PAA/PAH was cell attachment promoting for embryonic rat hippocampal and NG108-15 cells to a greater extent and somewhat for neonatal rat cardiac myocytes and C2C12 skeletal muscle cells. High fidelity beating patterns of neonatal rat cardiac myocytes were observed after 100 days on polyelectrolyte patterns in the serum-free medium For cardiac muscle cells, the serum-free plating medium consisted of Ultraculture, a general purpose medium, supplemented with different components as specified in the Materials and Methods, under the heading; Culture Methods (a) Cardiac myocytes. For embryonic hippocampal cells, the serum-free plating medium consisting of Neurobasal E medium supplemented with different components as specified in the Materials and Methods, under the heading; Culture Methods (b) Hippocampal cells. For NG108-15 cells, the serum-free differentiating medium consisted of DMEM and 2% B-27. as specified in the Materials and Methods, under the heading; Culture Methods (c) NG108-15 cells. For C2C12 cells, the cells were cultured and plated according to the same protocol as the NG108-15 cells with minor adaptations as specified in the Materials and Methods, under the heading; Culture Methods (d) C2C12 cells https://onlinelibrary.wiley.com/doi/pdf/10.1002/btpr.150 |
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Source | Literature - modified commercial product |
Chemically defined > Unspecified | Unspecified |