Cell line/type | C2C12 (skeletal muscle cell line) |
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Species | Mouse |
Animal free | Unspecified |
Product | Ult-G-AIM |
Fujita, H., Endo, A., Shimizu, K., & Nagamori, E. (2010). Evaluation of serumāfree differentiation conditions for C2C12 myoblast cells assessed as to active tension generation capability. Biotechnology and bioengineering, 107(5), 894-901. doi: 10.1002/bit.22865 In this study, several serum-free media for the differentiation of C2C12 myoblasts are compared and the extent of differentiation was assesed in several ways including as to active tension generation capability. C2C12 cells were allowed to differentiate in Dulbecco's modified Eagle's medium (DMEM) containing Ham's F-12 (F-12), AIM-V (AIM), 0.2% Ultroser-G in DMEM (Ult-G), and 0.1% Sericin in DMEM (Sericin), compared with in DMEM supplemented with 2% horse serum (HS) or 2% calf serum (CS). All serum-free media except for F-12 gave the sarcomere structure.All serum-free media examined were capable of inducing myotube formation and the expression of muscle-specific proteins. All serum-free media except for F-12 gave the sarcomere structure. The addition of Ult-G to the AIM resulted in improvement of the active tension generation capability, the active tension generated being approximately 3.4x compared to that in HS. Ult-G-AIM was most optimal serum-free medium among the media examined, which was even better than serum-containing media. Thus, Ult-G-AIM may replace serum-containing media for C2C12 study in the future. The approach for assessing muscle cell differentiation presented in this study will be suitable for other studies that involve the differentiation of muscle cells. The ingredients of Ultroser-G and AIM-V are not available. Their formulation is proprietary. https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.22865 |
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Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |