Cell line/type | BMC (Bone marrow cell) |
---|---|
Species | Mouse |
Animal free | Yes |
Product | StemPro MSC Xeno Free medium Iwamoto et al. 2013 |
Iwamoto, T., Terai, S., Hisanaga, T., Takami, T., Yamamoto, N., Watanabe, S., & Sakaida, I. (2013). Bone-marrow-derived cells cultured in serum-free medium reduce liver fibrosis and improve liver function in carbon-tetrachloride-treated cirrhotic mice. Cell and tissue research, 351(3), 487-495. The aim of this study is to derive liver repair cells from small amounts of autologous bone marrow aspirates obtained under local anesthesia and to use these cells in liver cirrhosis patients. By using a mouse model, basic research aimed at achieving novel liver regeneration therapy. Bone marrow cells aspirated from the femurs of C57 BL/6 Tg14 (act-EGFP) OsbY01 mice were cultured with serum-free medium (good manufacturing practice grade). The obtained spindle-shaped GFP-positive cells were injected (1x10(4) cells) via the caudal vein into mice with carbon tetrachloride (CCl4)-induced cirrhosis. Bone-marrow-derived cells cultured with a serum-free medium clearly had an effect on repairing liver fibrosis. These results are important for translational research on cultured bone-marrow-derived cell therapy for liver cirrhosis. Isolated GFP-positive BMCs were cultured on a CELL-start-coated dish in StemPro MSC Xeno Free medium with 2 mML-glutamine, StemPro LipoMax supplement and 100 μg/ml penicillin-streptomycin. StemPro MSC Xeno Free medium is a commercially available product. The precise formulation is proprietary. https://link.springer.com/content/pdf/10.1007%2Fs00441-012-1528-z.pdf |
|
Source | Literature - modified commercial product |
Chemically defined > Yes | Yes |
Antibiotics free > No | No |