Cell line/type | BM-MSCs (Bone marrow-derived mesenchymal stromal cells) |
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Species | Human |
Animal free | Unspecified |
Product | BD-SFM / MSX |
Gottipamula, S., Ashwin, K. M., Muttigi, M. S., Kannan, S., Kolkundkar, U., & Seetharam, R. N. (2014). Isolation, expansion and characterization of bone marrow-derived mesenchymal stromal cells in serum-free conditions. Cell and tissue research, 356(1), 123-135. In this study, serum-free isolation and expansion of MSCs is reported and compared with cells grown in conventional fetal bovine serum (FBS)-containing media as a control. BD Mosaic Mesenchymal Stem Cell Serum-Free media (BD-SFM) and Mesencult-XF (MSX) supported the isolation, sequential passaging, tri-lineage differentiation potential and acceptable surface marker expression profile of bone marrow-derived mesenchymal stromal cells (BM-MSCs). Further, MSCs cultured in SFM showed higher immune suppression and hypo-immunogenicity properties, making them an ideal candidate for allogeneic cell therapy. Although cells cultured in control media have a significantly higher proliferation rate, BM-MSCs cultured in BD-SFM or MSX media are the preferred choice to meet regulatory requirements as they do not contain bovine serum. While BM-MSCs cultured in BD-SFM and MSX media adhered to all MSC characteristics, in the case of few parameters, the performance of cells cultured in BD-SFM was superior to that of MSX media. BD Mosaic Mesenchymal Stem Cell Serum-Free media and Mesencult-XF are commercially available products. Their formulation is proprietary. https://link.springer.com/content/pdf/10.1007%2Fs00441-013-1783-7.pdf |
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Source | Literature - commercial |
Chemically defined > Yes | Yes |